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Registro Completo |
Biblioteca(s): |
Epagri-Sede. |
Data corrente: |
11/09/2018 |
Data da última atualização: |
11/09/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CARDOZA, Y. F.; ARAUJO, L.; DUARTE, V.; MORAES, M. G. |
Título: |
Identification of Pectobacterium carotovorum as the causal agent of bacterial canker on Pyrus communis L in Brazil. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS OF PLANT PATHOLOGY, 11., 2018, Boston, USA. Abstracts... Minneapolis - Minnesota - USA: American Phytopathological Society, 2018. p. 984 |
Idioma: |
Inglês |
Conteúdo: |
In June 2016, bacterial canker-like symptoms were observed on Pyrus communis L cv. Yali in one commercial orchard
in Monte Castelo, SC. Eight-hundred of 1.200 plants were lost by death and, finally, tree eradication. In affected trees,
the disease manifested as cankers with exudates on trunks and twigs, and the stems turned brown and slowly died.
Bacteria were isolated on King B medium from infected tissues. Biochemical tests including Biolog® GEN III were
conducted and some isolates were selected for whole genome sequencing throught Ion Torrent? platform. PCR with
Y1/Y2 and PcaF/PcaR primers, specific for P. carotovorum and P. carotovorum subsp. actinidiae respectively were
also carried out. A total of 15 bacterial colonies were isolated from infected tissues. All were gram-negative,
facultatively anaerobic, rod-shaped and exhibited a strong pectolitic activity in potato and carrot disks. Biolog assay
showed that the isolates were homogeneus, and three of them were selected for further tests. The organism with the
highest genome and proteome identity (99.8%) with the isolates was Pectobacterium carotovorum subsp. actinidiae. A
product of the expected size was obtained in both especific PCR. Inoculation of pear tree branches with 24h old
colonies showed that the bacterium was able to colonize the plant tissues. More tests, including kiwifruit inoculation,
are in progress to fulfill Koch?s postulates. To our knowledge, this is the first report of P. carotovorum causing pear
bacterial canker-like symptoms in Brazil. Identification and monitoring of this pathogen is very important due to large
economic losses that can cause MenosIn June 2016, bacterial canker-like symptoms were observed on Pyrus communis L cv. Yali in one commercial orchard
in Monte Castelo, SC. Eight-hundred of 1.200 plants were lost by death and, finally, tree eradication. In affected trees,
the disease manifested as cankers with exudates on trunks and twigs, and the stems turned brown and slowly died.
Bacteria were isolated on King B medium from infected tissues. Biochemical tests including Biolog® GEN III were
conducted and some isolates were selected for whole genome sequencing throught Ion Torrent? platform. PCR with
Y1/Y2 and PcaF/PcaR primers, specific for P. carotovorum and P. carotovorum subsp. actinidiae respectively were
also carried out. A total of 15 bacterial colonies were isolated from infected tissues. All were gram-negative,
facultatively anaerobic, rod-shaped and exhibited a strong pectolitic activity in potato and carrot disks. Biolog assay
showed that the isolates were homogeneus, and three of them were selected for further tests. The organism with the
highest genome and proteome identity (99.8%) with the isolates was Pectobacterium carotovorum subsp. actinidiae. A
product of the expected size was obtained in both especific PCR. Inoculation of pear tree branches with 24h old
colonies showed that the bacterium was able to colonize the plant tissues. More tests, including kiwifruit inoculation,
are in progress to fulfill Koch?s postulates. To our knowledge, this is the first report of P. carotovorum causing pear
b... Mostrar Tudo |
Palavras-Chave: |
apple diseases; bacterial diseases; malus domestica. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02357naa a2200193 a 4500 001 1127693 005 2018-09-11 008 2018 bl uuuu u00u1 u #d 100 1 $aCARDOZA, Y. F. 245 $aIdentification of Pectobacterium carotovorum as the causal agent of bacterial canker on Pyrus communis L in Brazil.$h[electronic resource] 260 $c2018 520 $aIn June 2016, bacterial canker-like symptoms were observed on Pyrus communis L cv. Yali in one commercial orchard in Monte Castelo, SC. Eight-hundred of 1.200 plants were lost by death and, finally, tree eradication. In affected trees, the disease manifested as cankers with exudates on trunks and twigs, and the stems turned brown and slowly died. Bacteria were isolated on King B medium from infected tissues. Biochemical tests including Biolog® GEN III were conducted and some isolates were selected for whole genome sequencing throught Ion Torrent? platform. PCR with Y1/Y2 and PcaF/PcaR primers, specific for P. carotovorum and P. carotovorum subsp. actinidiae respectively were also carried out. A total of 15 bacterial colonies were isolated from infected tissues. All were gram-negative, facultatively anaerobic, rod-shaped and exhibited a strong pectolitic activity in potato and carrot disks. Biolog assay showed that the isolates were homogeneus, and three of them were selected for further tests. The organism with the highest genome and proteome identity (99.8%) with the isolates was Pectobacterium carotovorum subsp. actinidiae. A product of the expected size was obtained in both especific PCR. Inoculation of pear tree branches with 24h old colonies showed that the bacterium was able to colonize the plant tissues. More tests, including kiwifruit inoculation, are in progress to fulfill Koch?s postulates. To our knowledge, this is the first report of P. carotovorum causing pear bacterial canker-like symptoms in Brazil. Identification and monitoring of this pathogen is very important due to large economic losses that can cause 653 $aapple diseases 653 $abacterial diseases 653 $amalus domestica 700 1 $aARAUJO, L. 700 1 $aDUARTE, V. 700 1 $aMORAES, M. G. 773 $tIn: INTERNATIONAL CONGRESS OF PLANT PATHOLOGY, 11., 2018, Boston, USA. Abstracts... Minneapolis - Minnesota - USA: American Phytopathological Society, 2018. p. 984
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Epagri-Sede (Epagri-Sede) |
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Registros recuperados : 17 | |
5. | | ARAUJO, L.; CARDOZA, Y. F.; DUARTE, V.; MORAES, M. G. Pseudomonas syringae causing bacterial canker on apple trees in Brazil. Bragantia, Campinas, SP, v. 79, n. 4, p. 1-7, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
Biblioteca(s): Epagri-Sede. |
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6. | | ARAUJO, L.; CARDOZA, Y. F.; DUARTE, V.; MORAES, M. G. Symptoms of bacterial shoot blight associated with Pseudomonas syringae pv. syringae in apple trees in Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife, PE. Resumos... Recife, PE: Sociedade Brasileira de Fitopatologia, 2019. p. 92Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Epagri-Sede. |
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9. | | ARAUJO, G. S. C.; SILVA, M. M.; RODRIGUES, M. L. G.; MORAES, M.; MONTEIRO, M.; VICTÓRIA, R.; CALEARO, D. Comportamento da pressão atmosférica e do vento máximo no episódio catarina: resultados preliminares. In: CONGRESSO BRASILEIRO DE METEOROLOGIA , 13., 2004, Fortaleza, CE. Meteorologia Tropical: [s.n.], 2004.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Epagri-Sede. |
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10. | | VALOIS, C. M.; COMIN, J. J.; VEIGA, M.; BRUNETTO, G.; FAYAD, J. A.; BAUER, F. C.; LOSS, A.; MORAES, M. P.; LAZZARI, C. J. R. Atributos físicos de Cambissolo Háplico em vinhedos submetidos a intensidades de tráfego. Revista Brasileira de Ciência do Solo, Viçosa, v. 38, n. 4, p. 1256-1267, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
Biblioteca(s): Epagri-Sede. |
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11. | | AMBROSINI, V. G.; BRUNETTO, G.; NAVA, G.; OLIVEIRA, B. S.; HEIZEN, J.; MORAES, M. P.; BERNARDINO, L.; MARIA, G. L.; SETE, P. B.; LAZZARI, C. Nível de suficiência de fósforo em folhas de pereira cv Rocha Pyrus communis L no Planalto Serrano de Santa Catarina. In: SEMINARIO NACIONAL SOBRE FRUTICULTURA DE CLIMA TEMPERADO, 10., 2012, São Joaquim, SC. Anais (Resumos de palestras e de pôsteres)... Florianópolis, SC: Epagri, 2012. p. 134.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Epagri-Sede. |
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12. | | BRUNETTO, G.; NAVA, G.; AMBROSINI, V. G.; OLIVEIRA, B. S.; HEINZEN, J.; MORAES, M. P.; CANTON, L.; SETE, P. B.; COMIN, J. J. Nível de suficiência de potássio em folhas de pereira cv Rocha no Planalto Serrano de Santa Catarina. In: SEMINRIO NACIONAL SOBRE FRUTICULTURA DE CLIMA TEMPERADO, 10., 2012, São Joaquim, SC. Anais (Resumos de palestras e de pôsteres)... Florianópolis, SC: Epagri, 2012. p. 135.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Epagri-Sede. |
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13. | | FREITAS, T. F. S.; HICKEL, E. R.; KHRIMIAN, A.; BORGES, M.; MICHEREFF, M. F. F.; BARRIGOSSI, J. A.; LAUMANN, R. A.; GUGGILAPU, S. D.; SANT’ANA, J.; BLASSIOLI-MORAES, M. C. Field Responses of Rice Stalk Stink Bug, Tibraca limbativentris, to Synthetic Sex Pheromone and Isomers of 1,10-Bisaboladien-3-ol. Neotropical Entomology, Londrina, v. 50, n. 2, p. 282-288, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
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14. | | BLASSIOLI-MORAES, M. C.; VENZON, M.; SILVEIRA, L. C. P.; GONTIJO, L. M.; TOGNI, P. H. B.; SUJII, E. R.; HARO, M. M.; BORGES, M.; MICHEREF, M. F. F.; AQUINO, M. F. S.; LAUMANN, R. A.; CAULFELD, J.; BIRKETT, M. Companion and Smart Plants: Scientifc Background to Promote Conservation Biological Control. Neotropical Entomology, New York, p. 1-17, 2022.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Epagri-Sede. |
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15. | | BLASSIOLI-MORAES, M. C.; KHRIMIAN, A.; MICHEREFF, M. F. F.; MAGALHÃES, D. M.; HICKEL, E. R.; FREITAS, T. F. S.; BARRIGOSSI, J. A. F.; LAUMANN, R. A.; SILVA, A. T.; GUGGLIAPU, S. D.; SILVA, C. C.; SANT'ANA, J.; BORGES, M. Male-Produced Sex Pheromone of Tibraca limbativentris Revisited: Absolute Configurations of Zingiberenol Stereoisomers and their Influence on Chemotaxis Behavior of Conspecific Female. Journal of Chemical Ecology, Heidelberg, p. 1-9, 2019.Tipo: Artigo em Anais de Congresso / Nota Técnica |
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16. | | CORREA, C. R. L.; ARAUJO, G. S. C.; CALEARO, D.; MORAES, M.; RODRIGUES, M. L. G.; MONTEIRO, M.; SILVA, M. M.; VICTÓRIA, R.; ROLDÃO, A.; ARAUJO, C. E. S. Monitoramento do catarina no centro operacional da Epagri/Climerh. In: CONGRESSO BRASILEIRO DE METEOROLOGIA , 13., 2004, Fortaleza, CE. Meteorologia Tropical: [s.n.], 2004.Tipo: Artigo em Anais de Congresso / Nota Técnica |
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17. | | BRUNO, J. B.; LIMA-VERDE, I. B.; MARTINS, F. S.; MATOS, M. H. T.; LOPES, C. A. P.; MAIA-JR., J. E.; BÁO, S. N.; NOBRE JUNIOR, H. V.; MAIA, F. D.; PESSOA, C.; MORAES, M. O.; SILVA, J. R. V.; FIGUEIREDO, J. R.; RODRIGUES, A. P. R. Característica histológica, ultra-estrutural e produção de nitrito de folículos pré-antrais caprinos cultivados in vitro na ausência ou presença de soro. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 60, n. 6, p. 1329-1337, 2008.Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - -- |
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Registros recuperados : 17 | |
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